Characterization and Production of Goat Milk Kefir-Peptide on Triglyceride Synthesis of Cell Model of 3T3-L1

Goat milk kefir (GMK) refers to fermented products, generated through fermenting with microbial culture called grains. Prior studies have reported the changes in quality properties during aging process which fermentation continues, resulting of peptide content. This research aims investigate effect time on GMK–peptide characteristics inhibite triglyceride syntheses cells model 3T3-L1. In this study, GMK were stored at 4 ± 1 oC for 0, 2, 4, 6 and 8 weeks, respectively. The protein profile was characterizedby implementing SDS-PAGE. result experiment indicated no degradation weeks period particularly high molecular weight 84 kDa, 80 kDa 65 kDa. GMK-supernatant from storage performed by applying ultrafiltration membrane cut off < 30 (UFC8 030.08). GMK-filtrate fractions (GMK-peptide) collected as peptides. After period, found consist amino acid. proteolytic activity grain increased linearly along (0-8 weeks). During released peptides particular, antioxidant activities significantly different (p<0.05) periods. GMK-peptide elevating concentration, 3.30 % 55.73%, derivate GMK-peptides 2.75 – 10.39 mg/ml. Obesity associated adipocyte hypertrophy occurred when TG accumulation.GMK-peptide 100 mg/ml lowest level (2.00 0.03 mg/dl). finding line inhibition synthesis (61.14 3.26 %). However, contained potency due may be corelated decreasing synthesis.The study thus suggested important role prolong generating higher bioactive cell 3T3-L1